Integrated Method for Single-Cell DNA Extraction, PCR Amplification, and Sequencing of Ribosomal DNA from Harmful Dinoflagellates Cochlodinium polykrikoides and Alexandrium catenella
نویسندگان
چکیده
منابع مشابه
An Efficient Method For DNA Extraction From Paraffin Wax Embedded Tissues For PCR Amplification Of Human And Viral DNA
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline sol...
متن کاملcomparison of five simple methods for dna extraction from echinococcus granulosus protoscoleces for pcr-amplification of ribosomal dna
background: cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and iran. since extraction of dna from the parasite is a primary and crucial step which has a principal effect on pcr results, in the current study five simple methods for dna extraction from protoscoleces of echinococcus granulos...
متن کاملan efficient method for dna extraction from paraffin wax embedded tissues for pcr amplification of human and viral dna
background and objective: formalin-fixed paraffin-embedded tissues are a valuable source of dna for molecular studies. we designed and optimized an efficient procedure for dna extraction from formalin-fixed paraffin embedded tissues. materials and methods: seventy three blocks of cervical paraffin-embedded tissues were investigated. dna was extracted using 45 minutes boiling in alkaline solutio...
متن کاملONE-STEP NUCLEIC ACID EXTRACTION AND AMPLIFICATION ON AN INTEGRATED MICRODEVICE FOR EARLY DETECTION OF Cochlodinium polykrikoides
Herein, a monolithic polycarbonate (PC) microdevice was developed to handle both DNA extraction and polymerase chain reaction (PCR) processes successively. Target cell – Cochlodinium polykrikoides microalgae, the causative species for harmful algal blooms in South Korea – was firstly captured on amine-functionalized microbeads via electrostatic interaction and then lysed to release DNA into sub...
متن کاملComparison of Five Simple Methods for DNA Extraction from Echinococcus granulosus Protoscoleces for PCR- Amplification of Ribosomal DNA
Background: Cystic hydatid disease is an important zoonosis, affecting humans and animals and is a significant public health and economic problem throughout the world and Iran. Since extraction of DNA from the parasite is a primary and crucial step which has a principal effect on PCR results, in the current study five simple methods for DNA extraction from protoscoleces of Echinococcus granulos...
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ژورنال
عنوان ژورنال: Marine Biotechnology
سال: 2004
ISSN: 1436-2228,1436-2236
DOI: 10.1007/s10126-004-1700-x